Advantages and disadvantages of the serial dilution agar plate technique

ADVANTAGES AND DISADVANTAGES OF THE SERIAL DILUTION AGAR PLATE TECHNIQUE SERIES

Study online flashcards and notes for Serial Dilution method including Advantages of serial dilution-agar plate: 1. The serial dilution is a simple process where a known amount of a cell stock culture is transferred to a new broth and tube to create a dilution.

Cryptococcal meningitis is an opportunistic infection caused by Cryptococcus neoformans (serotypes A and D) and Cryptococcus gattii (serotypes B and C).Cryptococcus neoformans is acquired by inhalation and causes pneumonia.Organize, analyze and graph and present your scientific data. This same technique can be used to dilute a liquid culture of bacteria. As illustrated here with a serial dilution of dye, each dilution contains less of the original sample.

ADVANTAGES AND DISADVANTAGES OF THE SERIAL DILUTION AGAR PLATE TECHNIQUE SERIES

A serial dilution is a series of successive measured dilutions.

Repeat the steps until the cells can be observed under the microscope when the diluted sample was observed. First, take a portion of the sample and does serial dilution on it.

Serial dilution is a simple yet efficient technique to determine the number of cells or organisms in a concentrated sample.

In this instance, a complete error budget calculation would probably show that the overall uncertainty at a coverage factor of 2 might be too large from. Bearing in mind that at a coverage factor, k, of 2 (95.45% Confidence), the volumetric uncertainty alone would approach 1%.

Ignoring the uncertainty contribution associated with weighing 0.5 mg of a reference standard, the volumetric uncertainty due to the serial dilution is 0.40%, which is 1.6 times larger than a simple 1 to 50 dilution.Definingthe acceptable accuracyofasusceptibilitytest is a more challenging task whenone considers the numerous Thecontentionthat the acceptableprecisionofan MICbased on dilution testing is a range of three twofold dilutions (target value + 1 dilution) nowseemswell estab-lished andsupportedbypioneering studies (1). Serial dilution is used in microbiology to estimate the concentration or number of cells/organisms in a sample to obtain an incubated plate with an easily countable number of colonies. Serial dilution is performed in a number of experimental sciences like biochemistry, pharmacology, physics, and homeopathy.

Advantages and disadvantages of the serial dilution agar plate technique